Reprinted from ]OUa.......AL OF IXVERTEBRATE PATHOLOGY

No occurrence of milky disease resulted when third-instal' larvae of the Japanese beetle, Popillia iaponica, were exposed to soil containing 2 billion spores/kg of strain NRRLB-2.309l'vI of tile milky disease bacterium, Bacillus popilliae, produced on a laboratory medium, or when the larvae imbibed 200,000 of the spores of strain NRRL B-2309M in aqueous suspension; however, 24% of the larvae were infected when they were injected with 10,000 of the spores. In conh'ast, spores produced conmlerciaIIy2 in diseased larvae infected 60% of the larvae exposed to soil containing 2 billion spores/kg, 60% of the larvae that imbibed 280,000 spores in an aqueous suspension, and 40% of the larvae injected with 10,000 spores.